Evans D, Jeske H
Institut für Allgemeine Botanik, Angewandte Molekular Biologie der Pflanzen, Hamburg, Germany.
Virology. 1993 Nov;197(1):492-6. doi: 10.1006/viro.1993.1619.
Mutations have been inserted into the complementary sense ORFs of DNA A of Abutilon mosaic virus (AbMV). Mutation in both ORF AC1 and ORF AC2/3 resulted in clones incapable of infecting Nicotiana benthamiana following agroinoculation. Mutation in ORF AC1 but not in ORF AC2/3 prevented DNA replication in leaf discs. Thus ORF AC1 is essential for AbMV DNA replication whereas ORF AC2/3 is probably involved in some aspect of the spread of the virus through the plant. Coagroinoculation of both mutants and wild-type DNA B resulted in complementation and recombination. Complementation precedes recombination and once recombination is established mutant DNA can no longer be detected. Plants in which complementation alone is occurring are symptomless and contain relatively low amounts of virus double-stranded DNA whereas following recombination a wild-type infection is established.
突变已被插入到苘麻花叶病毒（AbMV）DNA A的互补义开放阅读框中。ORF AC1和ORF AC2/3中的突变均导致克隆在农杆菌接种后无法感染本氏烟草。ORF AC1中的突变而非ORF AC2/3中的突变阻止了叶盘DNA的复制。因此，ORF AC1对AbMV DNA复制至关重要，而ORF AC2/3可能参与病毒在植物体内传播的某些方面。两种突变体与野生型DNA B共农杆菌接种导致互补和重组。互补先于重组，一旦重组发生，突变DNA就无法再被检测到。仅发生互补的植物无症状，且含有相对少量的病毒双链DNA，而重组后会建立野生型感染。
