噬菌体phi 6单链RNA基因组前体的体外包装

In vitro packaging of the bacteriophage phi 6 ssRNA genomic precursors.

作者信息

Gottlieb P, Strassman J, Frucht A, Qiao X Y, Mindich L

机构信息

Department of Microbiology, Public Health Research Institute New York, New York 10016.

出版信息

Virology. 1991 Apr;181(2):589-94. doi: 10.1016/0042-6822(91)90892-f.

DOI:10.1016/0042-6822(91)90892-f

PMID:2014638

Abstract

Bacteriophage phi 6 contains three segments of double-stranded RNA within a nucleocapsid. Plasmids containing cDNA copies of the large genomic segment direct the synthesis of viral proteins that assemble into procapsids in Escherichia coli or Pseudomonas phaseolicola. These structures are dodecahedral assemblages of proteins P1, P2, P4, and P7. We report in this paper that these particles are capable of packaging viral single-stranded plus-sense RNA in vitro. The packaging reaction requires the presence of ATP or dATP. Synthesis of minus strands takes place within this filled procapsid in the presence of all four nucleoside triphosphates. Packaged ssRNA is found to be protected from added ribonuclease.

摘要

噬菌体φ6在核衣壳内包含三段双链RNA。含有大基因组片段cDNA拷贝的质粒指导病毒蛋白的合成，这些蛋白在大肠杆菌或菜豆假单胞菌中组装成原衣壳。这些结构是由蛋白质P1、P2、P4和P7组成的十二面体聚集体。我们在本文中报道，这些颗粒能够在体外包装病毒单链正链RNA。包装反应需要ATP或dATP的存在。在所有四种核苷三磷酸存在的情况下，负链在这种充满的原衣壳内合成。发现包装好的单链RNA受到保护，免受添加的核糖核酸酶的影响。