Mindich L, Qiao X, Qiao J
Department of Microbiology, Public Health Research Institute, New York, New York 10016, USA.
Virology. 1995 Sep 10;212(1):213-7. doi: 10.1006/viro.1995.1470.
Bacteriophage phi 6 has a genome of three segments of double-stranded RNA enclosed in a polyhedral procapsid. The preformed procapsid is capable of packaging plus-strand transcripts of the genomic segments in an in vitro reaction. Packaging of individual segments is dependent upon unique packaging sequences of about 300 nucleotides near the 5' ends of the segments. We have prepared segments L, M, and S with internal deletions that decrease their size by as much as sixfold without affecting either their packaging sequences or their 3' ends. Although packaging of genomic segments is normally very precise, with only one of each in a procapsid, these smaller segments are packaged in multiples such that the total number of nucleotides for each segment class approaches that of the normal genomic segment.
噬菌体φ6具有由三段双链RNA组成的基因组，这些RNA被包裹在一个多面体原衣壳中。预先形成的原衣壳能够在体外反应中包装基因组片段的正链转录本。单个片段的包装取决于片段5'端附近约300个核苷酸的独特包装序列。我们制备了L、M和S片段，这些片段带有内部缺失，其大小减小了多达六倍，而不影响它们的包装序列或3'端。尽管基因组片段的包装通常非常精确，每个原衣壳中只有一个，但这些较小的片段会以多个形式被包装，使得每个片段类别的核苷酸总数接近正常基因组片段的核苷酸总数。
