Departamento de Bioquímica y Biología Molecular-A, Facultad de Veterinaria, Universidad de Murcia, E-30100 Murcia, Spain.
Langmuir. 2010 Jun 1;26(11):8567-72. doi: 10.1021/la904637k.
A succinoyl trehalose lipid produced by Rhodococcus sp. behaves as a biological surfactant and also displays various interesting biological activities. Trehalose lipid has been shown to have a great tendency to partition into phospholipid membranes; therefore, the characterization of its interaction with biological membranes is of central importance. In this work, human red blood cells have been used as an experimental model. Trehalose lipid causes the swelling of human erythrocytes followed by hemolysis at concentrations well below its critical micellar concentration. Kinetic measurements show that, upon addition of trehalose lipid, K(+) release precedes that of hemoglobin. Osmotic protectants of the appropriate size added to the external medium make it possible to avoid hemolysis. The results indicate that trehalose lipid causes the hemolysis of human erythrocytes by a colloid-osmotic mechanism, most likely by formation of enhanced permeability domains, or "pores" enriched in the biosurfactant, within the erythrocyte membrane. Scanning electron microscopy shows trehalose lipid-induced spherocytosis and echinocytosis of red blood cells, which fits well within the framework of the bilayer-couple hypothesis. The presented results contribute to establishing a molecular basis for the biological properties of this trehalose lipid biosurfactant.
由红球菌属产生的琥珀酰化海藻糖脂作为一种生物表面活性剂，表现出各种有趣的生物活性。已经表明海藻糖脂具有很大的倾向分配到磷脂膜中; 因此，其与生物膜相互作用的特性具有重要的意义。在这项工作中，用人的红细胞作为实验模型。海藻糖脂在低于其临界胶束浓度的浓度下引起人红细胞的肿胀，随后发生溶血。动力学测量表明，在添加海藻糖脂后，钾（K +）释放先于血红蛋白释放。向外部介质中添加适当大小的渗透保护剂可以避免溶血。结果表明，海藻糖脂通过胶体渗透压机制导致人红细胞的溶血，很可能是通过在红细胞膜内形成富含生物表面活性剂的增强通透性区域或“孔”。扫描电子显微镜显示海藻糖脂诱导的红细胞球形和棘形，这与双层偶联假说非常吻合。所呈现的结果有助于为这种海藻糖脂生物表面活性剂的生物学特性建立分子基础。
