Ortiz Antonio, Teruel José A, Manresa Ángeles, Espuny María J, Marqués Ana, Aranda Francisco J
Departamento de Bioquímica y Biología Molecular-A, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, E-30100 Murcia, Spain.
Biochim Biophys Acta. 2011 Aug;1808(8):2067-72. doi: 10.1016/j.bbamem.2011.05.003. Epub 2011 May 10.
Bacterial trehalose lipids are biosurfactants with potential application in the biomedical/healthcare industry due to their interesting biological properties. Given the amphiphilic nature of trehalose lipids, the understanding of the molecular mechanism of their biological action requires that the interaction between biosurfactant and membranes is known. In this study we examine the interactions between a trehalose lipid from Rhodococcus sp. and dimyristoylphosphatidylglycerol membranes by means of differential scanning calorimetry, X-ray diffraction, infrared spectroscopy and fluorescence polarization. We report that there are extensive interactions between trehalose lipid and dimyristoylphosphatidylglycerol involving the perturbation of the thermotropic gel to liquid-crystalline phase transition of the phospholipid, the increase of fluidity of the phosphatidylglycerol acyl chains and dehydration of the interfacial region of the bilayer, and the modulation of the order of the phospholipid bilayer. The observations are interpreted in terms of structural perturbations affecting the function of the membrane that might underline the biological actions of the trehalose lipid.
细菌海藻糖脂是一类生物表面活性剂，因其有趣的生物学特性而在生物医学/医疗保健行业具有潜在应用价值。鉴于海藻糖脂的两亲性质，要了解其生物作用的分子机制，就需要知道生物表面活性剂与膜之间的相互作用。在本研究中，我们通过差示扫描量热法、X射线衍射、红外光谱和荧光偏振法，研究了红球菌属的一种海藻糖脂与二肉豆蔻酰磷脂酰甘油膜之间的相互作用。我们报告称，海藻糖脂与二肉豆蔻酰磷脂酰甘油之间存在广泛的相互作用，包括对磷脂热致凝胶向液晶相转变的扰动、磷脂酰甘油酰基链流动性的增加以及双层界面区域的脱水，以及对磷脂双层有序性的调节。这些观察结果是根据影响膜功能的结构扰动来解释的，这些扰动可能是海藻糖脂生物作用的基础。
