Kyoto Prefectural University of Medicine, Japan.
Int J Hyperthermia. 2010;26(2):101-7. doi: 10.3109/02656730903272917.
Previously we have demonstrated that whole body hyperthermia (WBH) improves insulin resistance in diabetic mice. The aim of the present study was to perform a gene expression analysis of the liver and adipose tissue of obesity-induced insulin resistant diabetic mice (db/db mice) after WBH and to define the molecules that play the important role in improvement of insulin resistance by WBH. Male db/db mice were treated with WBH 3 times per week for 12 weeks. Total RNA was extracted from the liver and adipose tissue of db/db mice, and differences in the gene expression profiles among db/+ mice, untreated db/db mice, and WBH-treated db/db mice were investigated using a high-density DNA microarray. WBH directly targets liver and adipose tissue, resulting in modifications in NF-kappaB and IL-6 signalling pathways, as well as lipid metabolism. Although the mechanisms have not yet been completely investigated, we can conclude that WBH may provide a new therapeutic or preventive modality against type 2 diabetes mellitus and metabolic or insulin resistance syndrome through the modification of several signalling pathways.
先前我们已经证明全身热疗（WBH）可以改善糖尿病小鼠的胰岛素抵抗。本研究的目的是对肥胖诱导的胰岛素抵抗糖尿病（db/db 小鼠）的肝脏和脂肪组织进行基因表达分析，确定 WBH 改善胰岛素抵抗的重要作用分子。雄性 db/db 小鼠每周接受 WBH 治疗 3 次，共 12 周。从 db/db 小鼠的肝脏和脂肪组织中提取总 RNA，并使用高密度 DNA 微阵列研究 db/+ 小鼠、未经处理的 db/db 小鼠和 WBH 处理的 db/db 小鼠之间的基因表达谱差异。WBH 直接靶向肝脏和脂肪组织，导致 NF-κB 和 IL-6 信号通路以及脂质代谢的改变。尽管机制尚未完全研究，但我们可以得出结论，WBH 可能通过几种信号通路的改变为 2 型糖尿病和代谢或胰岛素抵抗综合征提供新的治疗或预防方式。
