Kokura Satoshi, Adachi Satoko, Manabe Emiko, Mizushima Katsura, Hattori Takeshi, Okuda Toshimitsu, Nakabe Nami, Handa Osamu, Takagi Tomohisa, Naito Yuji, Yoshida Norimasa, Yoshikawa Toshikazu
Biomedical Safety Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
Int J Hyperthermia. 2007 May;23(3):259-65. doi: 10.1080/02656730601176824.
In this study, we examined the efficacy of whole body hyperthermia (WBH) on obesity-induced insulin resistance in diabetic mice.
Male db/db mice were treated with WBH 3 times per week for 12 weeks. The rectal temperature of mice reached 38.0 degrees C 5 min after heating, and was kept at 38.0 degrees C for 30 min. At the end of each week, tail snip glucose levels were determined under fasting conditions. The GLUT-4 gene expression of muscle tissue was analyzed by real-time PCR.
(1) WBH-treated db/db mice showed a significant decrease in fasting blood glucose level as compared with untreated db/db mice (p < 0.01). (2) Plasma insulin levels in untreated db/db mice at the age of 10 weeks were significantly increased compared with those of db/+ mice (p < 0.0001). On the other hand, the reduction (31%) in insulin levels in WBH-treated mice indicated improved insulin sensitivity. (3) The ability of WBH to increase insulin sensitivity was further established in glucose tolerance tests and insulin tolerance tests. (4) Urine albumin of db/db mice significantly increased compared with those of db/+ mice at 18 weeks of age (p < 0.001). This increase in urinary albumin was significantly inhibited by WBH (p < 0.01). (5) WBH up-regulated the expression of GLUT4 mRNA in skeletal muscle.
Although the mechanisms have not yet been completely investigated, WBH may provide a new therapeutic or preventive modality against obesity-related diseases such as T2DM and metabolic or insulin resistance syndrome.
在本研究中，我们检测了全身热疗（WBH）对糖尿病小鼠肥胖诱导的胰岛素抵抗的疗效。
雄性db/db小鼠每周接受3次WBH治疗，共12周。加热5分钟后小鼠直肠温度达到38.0摄氏度，并保持在38.0摄氏度30分钟。每周结束时，在禁食条件下测定尾尖血糖水平。通过实时PCR分析肌肉组织中GLUT-4基因的表达。
（1）与未治疗的db/db小鼠相比，接受WBH治疗的db/db小鼠空腹血糖水平显著降低（p<0.01）。（2）10周龄未治疗的db/db小鼠血浆胰岛素水平与db/+小鼠相比显著升高（p<0.0001）。另一方面，接受WBH治疗的小鼠胰岛素水平降低（31%）表明胰岛素敏感性提高。（3）在葡萄糖耐量试验和胰岛素耐量试验中进一步证实了WBH增加胰岛素敏感性的能力。（4）18周龄时，db/db小鼠的尿白蛋白与db/+小鼠相比显著增加（p<0.001）。WBH显著抑制了尿白蛋白的这种增加（p<0.01）。（5）WBH上调了骨骼肌中GLUT4 mRNA的表达。
尽管其机制尚未完全研究清楚，但WBH可能为对抗肥胖相关疾病如2型糖尿病和代谢或胰岛素抵抗综合征提供一种新的治疗或预防方式。
