Department of Biochemistry, University of Cambridge, Cambridge, UK.
FEMS Microbiol Lett. 2010 Mar;304(2):195-202. doi: 10.1111/j.1574-6968.2010.01901.x. Epub 2010 Jan 18.
The Pectobacterium atrosepticum strain SCRI1043 genome contains two complete prophage sequences. One, ECA41, is Mu-like and is able to integrate into, and excise from, various genomic locations. The other, ECA29, is a P2 family prophage, and is also able to excise from the genome. Excision of both prophages is rare and we were unable to induce lysis of cultures. Deletion of the entire prophages, both separately and in combination, did not affect the growth rate or the secretion of plant cell wall-degrading enzymes, but swimming motility was decreased. The virulence of prophage deletion strains in the potato host was decreased.
软腐果胶杆菌 SCRI1043 菌株的基因组包含两个完整的噬菌体序列。一个是 Mu 样的 ECA41，能够整合到基因组的不同位置并进行切除。另一个是 P2 家族的 ECA29，也能够从基因组中切除。这两个噬菌体的切除都很少见，我们也无法诱导培养物裂解。单独或组合删除整个噬菌体序列都不会影响生长速率或植物细胞壁降解酶的分泌，但泳动性会降低。噬菌体缺失菌株在马铃薯宿主中的毒力降低。
