Poynter Greg, Huss David, Lansford Rusty
Division of Biology and Biological Imaging Center, Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA.
Cold Spring Harb Protoc. 2009 Jan;2009(1):pdb.prot5118. doi: 10.1101/pdb.prot5118.
This protocol describes how to generate transgenic quail by injecting a lentiviral vector into freshly laid Japanese quail eggs at stage X. The lentivirus infects primordial germ cells originating in the area pellucida.
本方案描述了如何通过在X期将慢病毒载体注射到刚产下的日本鹌鹑蛋中来生成转基因鹌鹑。慢病毒感染起源于透明带区域的原始生殖细胞。
