将慢病毒注射到X期胚盘以生产转基因鹌鹑。
Injection of lentivirus into stage-X blastoderm for the production of transgenic quail.
作者信息
Poynter Greg, Huss David, Lansford Rusty
机构信息
Division of Biology and Biological Imaging Center, Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA.
出版信息
Cold Spring Harb Protoc. 2009 Jan;2009(1):pdb.prot5118. doi: 10.1101/pdb.prot5118.
PMID:20147013
Abstract
This protocol describes how to generate transgenic quail by injecting a lentiviral vector into freshly laid Japanese quail eggs at stage X. The lentivirus infects primordial germ cells originating in the area pellucida.
摘要
本方案描述了如何通过在X期将慢病毒载体注射到刚产下的日本鹌鹑蛋中来生成转基因鹌鹑。慢病毒感染起源于透明带区域的原始生殖细胞。
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