Intarapat Sittipon, Stern Claudio D
Department of Cell and Developmental Biology and UCL Centre for Stem Cells and Regenerative Medicine, University College London, Gower Street, London WC1E 6BT, UK.
Stem Cell Res. 2013 Nov;11(3):1378-92. doi: 10.1016/j.scr.2013.09.005. Epub 2013 Sep 24.
Chick embryonic stem cells (cESCs) can be derived from cells obtained from stage X embryos (blastoderm stage); these have the ability to contribute to all somatic lineages in chimaeras, but not to the germ line. However, lines of stem cells that are able to contribute to the germ line can be established from chick primordial germ cells (cPGCs) and embryonic germ cells (cEGCs). This review provides information on avian stem cells, emphasizing different sources of cells and current methods for derivation and culture of pluripotent cells from chick embryos. We also review technologies for isolation and derivation of chicken germ cells and the production of transgenic birds.
鸡胚胎干细胞（cESCs）可从X期胚胎（胚盘期）获得的细胞中分离得到；这些细胞能够在嵌合体中分化为所有体细胞谱系，但不能分化为生殖系。然而，能够分化为生殖系的干细胞系可从鸡原始生殖细胞（cPGCs）和胚胎生殖细胞（cEGCs）中建立。本综述提供了有关禽类干细胞的信息，重点介绍了不同的细胞来源以及从鸡胚胎中获取和培养多能细胞的当前方法。我们还综述了鸡生殖细胞的分离和获取技术以及转基因鸡的生产。
