丽蝇蛹集金小蜂（寄生蜂）的采卵
Egg collection for Nasonia (parasitoid wasp).
作者信息
Werren John H, Loehlin David W
机构信息
Department of Biology, University of Rochester, Rochester, NY 14627, USA.
出版信息
Cold Spring Harb Protoc. 2009 Oct;2009(10):pdb.prot5309. doi: 10.1101/pdb.prot5309.
Nasonia is a complex of four closely related species of wasps with several features that make it an excellent system for a variety of genetic studies. These include a short generation time, ease of rearing, interfertile species, visible and molecular markers, and a sequenced genome. Furthermore, its parasitoid lifestyle allows investigations of questions relating to parasitoid/host dynamics, host preference, and specialist versus generalist biology. It also can serve as a behavior model for studies of courtship, male aggression and territoriality, female dispersal, and sex ratio control. This protocol describes methods to count and/or collect Nasonia eggs. Fly hosts are placed in a foam plug such that only one oviposition site is available to the female wasp.
丽蝇蛹集金小蜂是一个由四种亲缘关系密切的黄蜂物种组成的复合体，具有多种特性，使其成为各种遗传学研究的理想系统。这些特性包括世代周期短、易于饲养、物种间可育、具有可见和分子标记以及已测序的基因组。此外，其寄生性生活方式有助于研究与寄生蜂/宿主动态、宿主偏好以及专性寄生与兼性寄生生物学相关的问题。它还可以作为研究求偶、雄性攻击和领地行为、雌性扩散以及性别比例控制的行为模型。本方案描述了对丽蝇蛹集金小蜂卵进行计数和/或收集的方法。将蝇类宿主放置在一个泡沫塞中，使雌性黄蜂只有一个产卵位点。
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