Werren John H, Loehlin David W
Department of Biology, University of Rochester, Rochester, NY 14627, USA.
Cold Spring Harb Protoc. 2009 Oct;2009(10):pdb.prot5308. doi: 10.1101/pdb.prot5308.
Nasonia is a complex of four closely related species of wasps with several features that make it an excellent system for a variety of genetic studies. These include a short generation time, ease of rearing, interfertile species, visible and molecular markers, and a sequenced genome. It also can serve as a behavior model for studies of courtship, male aggression and territoriality, female dispersal, and sex ratio control. Nasonia vitripennis is a parasitoid of a number of calliphorid flies, such as Sarcophaga bullata, allowing investigations of questions relating to parasitoid/host dynamics, host preference, and specialist versus generalist biology. S. bullata are relatively large, increasing the offspring yield that a single N. vitripennis female can produce. They are also easily reared in the lab if proper ventilation is available. This protocol describes the methods to collect and raise S. bullata larvae that will be used as hosts for N. vitripennis.
丽蝇蛹集金小蜂是由四种亲缘关系密切的黄蜂组成的复合体，具有多种特性，使其成为进行各种遗传学研究的理想系统。这些特性包括世代周期短、易于饲养、物种间可育、具有可见和分子标记以及已测序的基因组。它还可以作为研究求偶行为、雄性攻击和领地行为、雌性扩散以及性别比例控制的行为模型。丽蝇蛹集金小蜂是多种丽蝇科苍蝇（如红头丽蝇）的寄生蜂，这使得人们能够研究与寄生蜂/宿主动态、宿主偏好以及专性与广性生物学相关的问题。红头丽蝇体型相对较大，可提高单只丽蝇蛹集金小蜂雌性产生的后代数量。如果有适当的通风条件，它们也很容易在实验室中饲养。本方案描述了收集和饲养将用作丽蝇蛹集金小蜂宿主的红头丽蝇幼虫的方法。
