Werren John H, Loehlin David W
Department of Biology, University of Rochester, Rochester, NY 14627, USA.
Cold Spring Harb Protoc. 2009 Oct;2009(10):pdb.prot5307. doi: 10.1101/pdb.prot5307.
Nasonia is a complex of four closely related species of wasps with several features that make it an excellent system for a variety of genetic studies. These include a short generation time, ease of rearing, interfertile species, visible and molecular markers, and a sequenced genome. Furthermore, its parasitoid lifestyle allows investigations of questions relating to parasitoid/host dynamics, host preference, and specialist versus generalist biology. It also can serve as a behavior model for studies of courtship, male aggression and territoriality, female dispersal, and sex ratio control. This protocol describes standard rearing of Nasonia vitripennis strains on Sarcophaga bullata hosts.
丽蝇蛹集金小蜂是由四种亲缘关系密切的黄蜂组成的复合体，具有多种特性，使其成为进行各种遗传学研究的理想体系。这些特性包括世代周期短、易于饲养、物种间可育、具有可见标记和分子标记，以及拥有已测序的基因组。此外，其寄生性生活方式有助于研究与寄生蜂/宿主动态、宿主偏好以及专性与广性生物学相关的问题。它还可以作为求偶、雄性攻击和领地行为、雌性扩散以及性别比例控制研究的行为模型。本方案描述了在麻蝇宿主上对丽蝇蛹集金小蜂品系进行标准饲养的方法。
