利用T-DNA诱变技术转化小立碗藓 - Suppr

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Transformation of the moss Physcomitrella patens using T-DNA mutagenesis.

作者信息

Cove David J, Perroud Pierre-François, Charron Audra J, McDaniel Stuart F, Khandelwal Abha, Quatrano Ralph S

机构信息

Department of Biology, Washington University, St. Louis, MO 63130, USA.

出版信息

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5144. doi: 10.1101/pdb.prot5144.

DOI:10.1101/pdb.prot5144

PMID:20147074

Abstract

摘要

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相似文献

Transformation of the moss Physcomitrella patens using T-DNA mutagenesis.

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5144. doi: 10.1101/pdb.prot5144.

Transformation of the moss Physcomitrella patens using direct DNA uptake by protoplasts.

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5143. doi: 10.1101/pdb.prot5143.

Isolation and regeneration of protoplasts of the moss Physcomitrella patens.

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5140. doi: 10.1101/pdb.prot5140.

Somatic hybridization in the moss Physcomitrella patens using PEG-induced protoplast fusion.

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5141. doi: 10.1101/pdb.prot5141.

The moss Physcomitrella patens: a novel model system for plant development and genomic studies.

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.emo115. doi: 10.1101/pdb.emo115.

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Methods Mol Biol. 2014;1132:427-37. doi: 10.1007/978-1-62703-995-6_29.

High-level expression of secreted complex glycosylated recombinant human erythropoietin in the Physcomitrella Delta-fuc-t Delta-xyl-t mutant.

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Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5145. doi: 10.1101/pdb.prot5145.

Knocking out the wall: protocols for gene targeting in Physcomitrella patens.

Methods Mol Biol. 2011;715:273-90. doi: 10.1007/978-1-61779-008-9_19.

Molecular tools to study Physcomitrella patens.

Plant Biol (Stuttg). 2005 May;7(3):220-7. doi: 10.1055/s-2005-865645.

引用本文的文献

Targeted Gene Knockouts by Protoplast Transformation in the Moss .

Front Genome Ed. 2021 Dec 17;3:719087. doi: 10.3389/fgeed.2021.719087. eCollection 2021.

CRISPR/Cas9-Mediated Knockout of Physcomitrella patens Phytochromes.

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Curr Opin Plant Biol. 2018 Aug;44:175-180. doi: 10.1016/j.pbi.2018.04.019. Epub 2018 Jul 4.

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Plant Cell Physiol. 2018 Apr 1;59(4):651-660. doi: 10.1093/pcp/pcx182.