通过原生质体直接摄取DNA对小立碗藓进行转化。 - Suppr

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Transformation of the moss Physcomitrella patens using direct DNA uptake by protoplasts.

作者信息

Cove David J, Perroud Pierre-François, Charron Audra J, McDaniel Stuart F, Khandelwal Abha, Quatrano Ralph S

机构信息

Department of Biology, Washington University, St. Louis, MO 63130, USA.

出版信息

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5143. doi: 10.1101/pdb.prot5143.

DOI:10.1101/pdb.prot5143

PMID:20147073

Abstract

摘要

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Transformation of the moss Physcomitrella patens using direct DNA uptake by protoplasts.

作者信息

Cove David J, Perroud Pierre-François, Charron Audra J, McDaniel Stuart F, Khandelwal Abha, Quatrano Ralph S

机构信息

Department of Biology, Washington University, St. Louis, MO 63130, USA.

出版信息

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5143. doi: 10.1101/pdb.prot5143.

DOI:10.1101/pdb.prot5143

PMID:20147073

Abstract

摘要

相似文献

Transformation of the moss Physcomitrella patens using direct DNA uptake by protoplasts.

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5143. doi: 10.1101/pdb.prot5143.

Somatic hybridization in the moss Physcomitrella patens using PEG-induced protoplast fusion.

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5141. doi: 10.1101/pdb.prot5141.

Transformation of the moss Physcomitrella patens using T-DNA mutagenesis.

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5144. doi: 10.1101/pdb.prot5144.

Plastid transformation in Physcomitrella patens.

Methods Mol Biol. 2014;1132:427-37. doi: 10.1007/978-1-62703-995-6_29.

The moss Physcomitrella patens: a novel model system for plant development and genomic studies.

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.emo115. doi: 10.1101/pdb.emo115.

Knocking out the wall: protocols for gene targeting in Physcomitrella patens.

Methods Mol Biol. 2011;715:273-90. doi: 10.1007/978-1-61779-008-9_19.

Transformation of moss Physcomitrella patens gametophytes using a biolistic projectile delivery system.

Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5145. doi: 10.1101/pdb.prot5145.

High-level expression of secreted complex glycosylated recombinant human erythropoietin in the Physcomitrella Delta-fuc-t Delta-xyl-t mutant.

Plant Biotechnol J. 2007 May;5(3):389-401. doi: 10.1111/j.1467-7652.2007.00248.x. Epub 2007 Mar 15.

Gene function analysis by artificial microRNAs in Physcomitrella patens.

Methods Mol Biol. 2011;744:57-79. doi: 10.1007/978-1-61779-123-9_5.

Plastid Transformation in Physcomitrium (Physcomitrella) patens: An Update.

Methods Mol Biol. 2021;2317:321-331. doi: 10.1007/978-1-0716-1472-3_19.

引用本文的文献

The Antarctic moss 2-oxoglutarate/Fe(II)-dependent dioxygenases (Pn2-ODD2) enhanced the tolerance to drought and oxidative stress.

BMC Plant Biol. 2025 Apr 28;25(1):549. doi: 10.1186/s12870-025-06578-8.

Aquorin Bioluminescence-Based Ca Imaging Reveals Differential Calcium Signaling Responses to Abiotic Stresses in .

Plants (Basel). 2025 Apr 10;14(8):1178. doi: 10.3390/plants14081178.

A Moss 2-Oxoglutarate/Fe(II)-Dependent Dioxygenases (2-ODD) Gene of Flavonoids Biosynthesis Positively Regulates Plants Abiotic Stress Tolerance.

Front Plant Sci. 2022 Jul 29;13:850062. doi: 10.3389/fpls.2022.850062. eCollection 2022.

A minus-end directed kinesin motor directs gravitropism in Physcomitrella patens.

Nat Commun. 2021 Jul 22;12(1):4470. doi: 10.1038/s41467-021-24546-2.

Area from image analyses accurately estimates dry-weight biomass of juvenile tissue from the moss .

Appl Plant Sci. 2021 Apr 30;9(4):e11418. doi: 10.1002/aps3.11418. eCollection 2021 Apr.

Transfection of Arctic sp. KMR5045 as a Model for Genetic Engineering of Cold-Tolerant Mosses.

Front Plant Sci. 2021 Jan 8;11:609847. doi: 10.3389/fpls.2020.609847. eCollection 2020.

Mutations in the gene encoding Aminodeoxychorismate Synthase confer auxotrophic phenotypes.

MicroPubl Biol. 2021 Jan 26;2021. doi: 10.17912/micropub.biology.000364.

Multiplex CRISPR-Cas9 mutagenesis of the phytochrome gene family in Physcomitrium (Physcomitrella) patens.

Plant Mol Biol. 2021 Nov;107(4-5):327-336. doi: 10.1007/s11103-020-01103-x. Epub 2020 Dec 21.

Functional expression and characterization of cinnamic acid 4-hydroxylase from the hornwort Anthoceros agrestis in Physcomitrella patens.

Plant Cell Rep. 2020 May;39(5):597-607. doi: 10.1007/s00299-020-02517-z. Epub 2020 Feb 13.

CRISPR/Cas9-Mediated Knockout of Physcomitrella patens Phytochromes.

Methods Mol Biol. 2019;2026:237-263. doi: 10.1007/978-1-4939-9612-4_20.