Gharbiah Maey, Cooley James, Leise Esther M, Nakamoto Ayaki, Rabinowitz Jeremy S, Lambert J David, Nagy Lisa M
Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA.
Cold Spring Harb Protoc. 2009 Apr;2009(4):pdb.prot5188. doi: 10.1101/pdb.prot5188.
The marine gastropod Ilyanassa obsoleta is a long-standing and very useful model for studies of embryonic development. It is an especially important model for spiralian development, and for studies of asymmetric cell division. The embryos are amenable to classic embryological manipulation techniques as well as a growing number of molecular approaches. Ilyanassa is also an important model for studies of metamorphosis, the ecology of parasitism, the effects of environmental contaminants on morphology and sexual function, and comparative neurobiology. This protocol describes the procedure for extracting protein from Ilyanassa embryos for use in techniques such as Western blotting or two-dimensional (2D) gel electrophoresis.
海洋腹足纲动物光壳海笋是胚胎发育研究中一个长期且非常有用的模型。它是螺旋动物发育以及不对称细胞分裂研究中尤为重要的模型。其胚胎适用于经典的胚胎学操作技术以及越来越多的分子方法。光壳海笋也是变态发育、寄生生态学、环境污染物对形态和性功能的影响以及比较神经生物学研究的重要模型。本方案描述了从光壳海笋胚胎中提取蛋白质以用于蛋白质免疫印迹或二维（2D）凝胶电泳等技术的步骤。
