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锦龟卵的孵化及胚胎采集。

Egg incubation and collection of painted turtle embryos.

作者信息

Valenzuela Nicole

机构信息

Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011, USA.

出版信息

Cold Spring Harb Protoc. 2009 Jul;2009(7):pdb.prot5238. doi: 10.1101/pdb.prot5238.

DOI:10.1101/pdb.prot5238
PMID:20147203
Abstract

This protocol describes how to incubate painted turtle eggs and collect embryos for gene expression analysis. The same basic protocol can be used to incubate eggs and collect embryos from other turtle species by modifying the incubation temperature to suit the particular developmental needs of each species.

摘要

本方案描述了如何孵化锦龟卵并收集胚胎用于基因表达分析。通过调整孵化温度以适应每个物种的特定发育需求，相同的基本方案可用于孵化其他龟种的卵并收集胚胎。

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