Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA.
Bioinformatics. 2010 Apr 1;26(7):855-9. doi: 10.1093/bioinformatics/btq047. Epub 2010 Feb 9.
We conducted a reanalysis of genome-wide histone H3 tail methylation data in mammalian pluripotent and differentiated cells. We show that the promoters marked with histone H3 lysine 27 trimethylation (H3K27me3) tend to have more exonic positions in the promoter regions. However, this is not due to any preferential marking on exons over introns by H3K27me3. The relationship is also independent the status of histone H3 lysine 4 trimethylation (H3K4me3) mark, CpG content and the platforms used in the high-throughput profiling of histone modifications. It provides evidence for the link between histone modifications and transcribed exons in promoter regions.
我们对哺乳动物多能性和分化细胞中的全基因组组蛋白 H3 尾部甲基化数据进行了重新分析。结果表明，组蛋白 H3 赖氨酸 27 三甲基化（H3K27me3）标记的启动子在启动子区域中往往具有更多的外显子位置。然而，这并不是由于 H3K27me3 对外显子的优先标记造成的。这种关系也不依赖于组蛋白 H3 赖氨酸 4 三甲基化（H3K4me3）标记、CpG 含量以及在组蛋白修饰的高通量分析中使用的平台的状态。这为启动子区域中组蛋白修饰与转录外显子之间的联系提供了证据。
