Department of Ophthalmology, Harbin Medical University, 2nd Affiliated Hospital, Harbin City, Heilongjiang Province, People's Republic of China.
J Ocul Pharmacol Ther. 2010 Feb;26(1):11-9. doi: 10.1089/jop.2009.0037.
To evaluate expression changes in retina neurotrophic factors (NTs) in glaucomatous rats with copolymer-1 (COP-1) immunization.
Three episcleral veins in rat eyes were cauterized to produce chronic high intraocular pressure (IOP). Qualified rats were divided into 3 groups: glaucoma group, COP-1 immunization group, and PBS control group. Using immunohistochemistry and RT-PCR, we compared the expression of retinal NTs in these 3 groups.
NGF, BDNF, and NT-4 expression increased to different degrees and the levels of their RNAs had transient increases under elevated IOP, but there were no statistical differences with respect to the RNAs of interest in the COP-1 immunization group compared with the 2 control groups. Expression of NT-3 appeared earlier and stronger in the rats immunized with COP-1 than in rats with glaucoma. Moreover, the expression of the COP-1 receptor, TrkC, increased accordingly.
COP-1 immunization might increase NT-3 expression by some unknown mechanism in high IOP rats and the change may be related to COP-1 therapeutic intervention.
评估共聚物-1（COP-1）免疫治疗对青光眼大鼠视网膜神经营养因子（NTs）表达变化的影响。
通过灼烧大鼠眼 3 条巩膜静脉以产生慢性高眼压（IOP）。将合格的大鼠分为 3 组：青光眼组、COP-1 免疫组和 PBS 对照组。采用免疫组织化学和 RT-PCR 比较这 3 组视网膜 NTs 的表达。
NGF、BDNF 和 NT-4 的表达均不同程度地增加，IOP 升高时其 RNA 水平短暂增加，但 COP-1 免疫组与 2 个对照组相比，感兴趣的 RNA 没有统计学差异。与青光眼大鼠相比，COP-1 免疫大鼠中 NT-3 的表达出现得更早且更强。此外，COP-1 受体 TrkC 的表达相应增加。
COP-1 免疫可能通过某种未知机制增加高 IOP 大鼠中 NT-3 的表达，这种变化可能与 COP-1 的治疗干预有关。
