Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore.
Annu Rev Physiol. 2010;72:191-218. doi: 10.1146/annurev-physiol-021909-135840.
Many cellular signaling pathways ultimately control specific patterns of gene expression in the nucleus through a variety of signal-regulated transcription factors (TFs), including nuclear hormone receptors (NRs). The advent of genomic technologies for examining signal-regulated transcriptional responses and TF binding on a genomic scale has dramatically increased our understanding of the cellular programs that control hormonal signaling and gene regulation. Studies of TFs, especially NRs, using genomic approaches have revealed novel and unexpected features of hormone-regulated transcription, and a global view is beginning to emerge. In this review, we discuss the genomic methodologies that have been applied to the study of hormone-regulated gene expression, the results that have been obtained from using them, and the future prospects for these approaches. Given the wealth of information about hormone-dependent gene regulation by NRs, we have focused this review on the knowledge gained from genomic studies of their function.
许多细胞信号通路最终通过各种信号调节转录因子（TFs），包括核激素受体（NRs），来控制核内特定的基因表达模式。用于检测信号调节转录反应和 TF 在基因组范围内结合的基因组技术的出现，极大地提高了我们对控制激素信号和基因调控的细胞程序的理解。使用基因组方法研究 TFs，特别是 NRs，揭示了激素调节转录的新的和意外的特征，并且开始出现全局观点。在这篇综述中，我们讨论了已应用于研究激素调节基因表达的基因组方法、从使用这些方法获得的结果以及这些方法的未来前景。鉴于关于 NR 依赖激素的基因调控的信息量丰富，我们将本综述的重点放在通过基因组研究获得的关于它们功能的知识上。
