National Engineering Research Center for Miniaturized Detection Systems, Northwest University, Xi'an, Shaanxi, PR China.
Bioorg Chem. 2010 Jun;38(3):120-3. doi: 10.1016/j.bioorg.2010.01.003. Epub 2010 Jan 20.
The preparation of 1-deoxy-d-xylulose 5-phosphate, the key intermediate of MEP biosynthetic pathway for terpenoids by using recombinant 1-deoxy-d-xylulose 5-phosphate synthase of Rhodobacter capsulatus was optimized. The simple one-pot synthesis coupling with a newly established ion-exchange purification process affords the target compound with more than 80% yield and high purity (>95%). The procedure can also be employed to synthesize isotope labeled 1-deoxy-d-xylulose 5-phosphate by using isotope labeled starting materials.
利用来自荚膜红细菌（Rhodobacter capsulatus）的重组 1-脱氧-D-木酮糖 5-磷酸合酶，优化了萜类化合物甲羟戊酸途径关键中间产物 1-脱氧-D-木酮糖 5-磷酸的制备。通过新建立的离子交换纯化工艺，简单的一锅法合成可使目标化合物的产率超过 80%，纯度大于 95%。该方法还可用于使用同位素标记的起始原料合成同位素标记的 1-脱氧-D-木酮糖 5-磷酸。
