Hecht S, Kis K, Eisenreich W, Amslinger S, Wungsintaweekul J, Herz S, Rohdich F, Bacher A
Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstr. 4, D-85747 Garching, Germany.
J Org Chem. 2001 Jun 1;66(11):3948-52. doi: 10.1021/jo0100300.
Recombinant 1-deoxy-D-xylulose 5-phosphate synthase of Bacillus subtilis was used for the preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate using isotope-labeled glucose and/or isotope-labeled pyruvate as starting materials. The simple one-pot methods described afford almost every conceivable isotopomer of 1-deoxy-D-xylulose 5-phosphate carrying (13)C or (14)C from commercially available precursors with an overall yield around 50%.
枯草芽孢杆菌的重组1-脱氧-D-木酮糖5-磷酸合酶用于以同位素标记的葡萄糖和/或同位素标记的丙酮酸为起始原料制备同位素标记的1-脱氧-D-木酮糖5-磷酸。所述简单的一锅法能从市售前体中得到几乎每一种可想象的携带(13)C或(14)C的1-脱氧-D-木酮糖5-磷酸的同位素异构体，总产率约为50%。
