Medical and Molecular Parasitology Laboratory, University of Cologne, Medical School, Center of Anatomy, Institute II, Cologne, Germany.
Vet Parasitol. 2010 May 11;169(3-4):387-90. doi: 10.1016/j.vetpar.2010.01.014. Epub 2010 Jan 25.
Eighty fecal samples from pre-weaned calves with diarrhea were collected in the Tokachi area in Northern Japan to investigate the prevalence of Cryptosporidium species in such animals. Oocysts from fecal samples collected from each animal were concentrated using sucrose gradient centrifugation. Genomic DNA was extracted from each sample and processed by nested PCR to amplify the partial SSU rRNA gene of Cryptosporidium. Cryptosporidium infections were detected in 75% of the samples. Sequence analysis was performed on all positive samples. Phylogenetic analysis of 33 successfully sequenced isolates of the SSUrRNA PCR products revealed all but one were Cryptosporidium parvum infections. The remaining single case was Cryptosporidium bovis. These findings suggest that C. parvum is prevalent in diarrheic pre-weaned calves and can be a source of cryptosporidial infections for humans and animals in Hokkaido.
从日本北部十胜地区患有腹泻的未断奶小牛采集了 80 份粪便样本，以调查此类动物中隐孢子虫属物种的流行情况。使用蔗糖梯度离心从每只动物采集的粪便样本中浓缩卵囊。从每个样本中提取基因组 DNA，并通过巢式 PCR 处理以扩增隐孢子虫的部分 SSU rRNA 基因。在 75%的样本中检测到隐孢子虫感染。对所有阳性样本进行序列分析。对 33 个成功测序的 SSUrRNA PCR 产物的分离株进行系统发育分析显示，除 1 个外，其余均为微小隐孢子虫感染。剩下的单一病例为牛隐孢子虫。这些发现表明，微小隐孢子虫在腹泻性未断奶小牛中普遍存在，并且可能成为北海道人类和动物隐孢子虫感染的来源。
