State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Department of Veterinary Biosciences, Melbourne Veterinary School, The University of Melbourne, Parkville, VIC, Australia.
Parasit Vectors. 2017 Oct 25;10(1):519. doi: 10.1186/s13071-017-2463-3.
The protistan pathogens Cryptosporidium and Giardia can cause significant intestinal diseases in animals and humans. Cattle, particularly calves, carrying these protists can be significant reservoirs for human infections and disease. However, little is known about the genetic make-up of Cryptosporidium and Giardia populations in cattle and other ruminants in some regions of China.
In the present study, PCR-based tools were used to genetically characterise these protists in faecal samples from a total of 339 pre- and post-weaned calves from four distinct locations in Hubei Province using markers in the large (LSU) or small (SSU) subunits of nuclear ribosomal RNA genes. Cryptosporidium andersoni, C. bovis, C. ryanae and Giardia duodenalis assemblage E were detected in 0.6%, 10.9%, 4.1% and 22.6% of calves, respectively.
This study is the first to report the prevalence of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, and encourages large-scale molecular studies of animals and humans, in an effort to better understand the epidemiology of these enteric pathogens in China.
原生动物病原体隐孢子虫和贾第鞭毛虫可导致动物和人类发生严重的肠道疾病。携带这些原生动物的牛，尤其是小牛，可能是人类感染和疾病的重要传染源。然而，在中国的一些地区，对于牛和其他反刍动物中隐孢子虫和贾第鞭毛虫种群的遗传结构却知之甚少。
在本研究中，使用基于 PCR 的工具，针对来自湖北省四个不同地点的总共 339 头断奶前和断奶后的小牛的粪便样本，使用核核糖体 RNA 基因的大亚基（LSU）或小亚基（SSU）中的标记，对这些原生动物进行遗传特征分析。分别在 0.6%、10.9%、4.1%和 22.6%的小牛中检测到了隐孢子虫安氏、牛隐孢子虫、瑞亚隐孢子虫和贾第鞭毛虫 E 聚集。
本研究首次报道了湖北省断奶前和断奶后小牛中隐孢子虫和贾第鞭毛虫的流行情况，鼓励对动物和人类进行大规模的分子研究，以努力更好地了解这些肠道病原体在中国的流行病学。
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