Göncü Tuğrul, Tiryakioğlu Osman, Ozcan Ayhan, Ari Hasan, Sezen Mustafa, Vural Hakan, Bayer Ahmet, Yalçinkaya Serhat, Yavuz Senol, Ozyazicioğlu Ahmet
Department of Cardiovascular Surgery, Bursa Yüksek Ihtisas Education and Research Hospital, Bursa, Turkey.
Anadolu Kardiyol Derg. 2010 Feb;10(1):11-6. doi: 10.5152/akd.2010.004.
The purpose of this study was to compare the effects of ticlopidine and clopidogrel on the development of neointimal hyperplasia after experimental arterial injury.
This experimental, prospective, randomized controlled study was performed on twenty-seven rabbits, which were divided into three groups, each of which contained nine subjects. Following the development of a balloon catheter injury in the iliac artery, no drugs were administered to Group 1 (control). Group 2 was given ticlopidine, while Group 3 was given clopidogrel. At the end of the 21-day experimental period, arterial sections were evaluated histomorphologically and immunohistochemically with staining using antibodies against platelet derived growth factor beta and basic fibroblast growth factor. Statistical analyses were performed using Chi-Square, Mann Whitney U and one-way ANOVA tests.
At the end of study period, ticlopidine and clopidogrel strongly reduced the development of intimal hyperplasia after arterial injury (54.1%, p<0.001, 53.2%, p<0.001, respectively). No significant difference was observed in terms of intimal and medial areas between the drug-treated groups. Expressions of the basic fibroblast growth factor and platelet derived growth factor beta were significantly lower in the intima of drug treated groups with respect to the control group (p<0.05).
The results of our study suggest that ticlopidine and clopidogrel, which are widely used in antiplatelet treatment in clinics, can similarly prevent the development of intimal hyperplasia after experimental arterial injury.
本研究旨在比较噻氯匹定和氯吡格雷对实验性动脉损伤后新生内膜增生发展的影响。
本实验性、前瞻性、随机对照研究对27只兔子进行，将其分为三组，每组9只。在髂动脉造成球囊导管损伤后，第1组（对照组）不给予药物。第2组给予噻氯匹定，第3组给予氯吡格雷。在21天实验期结束时，对动脉切片进行组织形态学和免疫组织化学评估，使用抗血小板衍生生长因子β和碱性成纤维细胞生长因子的抗体进行染色。使用卡方检验、曼-惠特尼U检验和单因素方差分析进行统计分析。
在研究期结束时，噻氯匹定和氯吡格雷均强烈减少了动脉损伤后内膜增生的发展（分别为54.1%，p<0.001；53.2%，p<0.001）。在药物治疗组之间，内膜和中膜面积方面未观察到显著差异。与对照组相比，药物治疗组内膜中碱性成纤维细胞生长因子和血小板衍生生长因子β的表达显著降低（p<0.05）。
我们的研究结果表明，临床上广泛用于抗血小板治疗的噻氯匹定和氯吡格雷在实验性动脉损伤后可同样预防内膜增生的发展。
