Lee Patricia N, Callaerts Patrick, de Couet H Gert
Department of Zoology, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
Cold Spring Harb Protoc. 2009 Nov;2009(11):pdb.prot5323. doi: 10.1101/pdb.prot5323.
The ability to rear Hawaiian bobtail squid (Euprymna scolopes) embryos under controlled environmental conditions is a basic and necessary tool for developmental studies. It negates the need to know when fertilization occurred, allows correlation of the phase of development with the time of development (thereby facilitating collection of embryos at specific stages), and allows comparisons between cephalopod species. Embryonic development in E. scolopes is robust over a range of temperatures, is relatively rapid (approximately 21 d), and proceeds normally under laboratory conditions at ambient temperature (27 degrees C-29 degrees C). Here we present methods for maintaining E. scolopes embryos in culture from cleavage through hatching, as well as observing and recording live or freshly fixed embryos under a dissecting microscope.
在可控环境条件下培育夏威夷短尾乌贼（Euprymna scolopes）胚胎的能力，是发育研究的一项基本且必要的工具。它无需知道受精发生的时间，能将发育阶段与发育时间关联起来（从而便于在特定阶段收集胚胎），还能在头足类物种之间进行比较。在一定温度范围内，夏威夷短尾乌贼的胚胎发育强健，相对较快（约21天），并且在实验室环境温度（27摄氏度至29摄氏度）下能正常发育。在此，我们介绍从卵裂到孵化培养夏威夷短尾乌贼胚胎的方法，以及在解剖显微镜下观察和记录活体或刚固定胚胎的方法。
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