Department of Pathology, University of Michigan Medical School, 1301 Catherine Street, SPC 5602, Ann Arbor, MI 48109, USA.
Integr Cancer Ther. 2010 Mar;9(1):93-9. doi: 10.1177/1534735409360360. Epub 2010 Feb 11.
The purpose of this study was to determine whether a mineral-rich extract derived from the red marine algae Lithothamnion calcareum could be used as a dietary supplement for chemoprevention against colon polyp formation. A total of 60 C57bl/6 mice were divided into 3 groups based on diet. One group received a low-fat, rodent chow diet (AIN76A). The second group received a high-fat "Western-style" diet (HFWD). The third group was fed the same HFWD with the mineral-rich extract included as a dietary supplement. Mice were maintained on the respective diets for 15 months. Autopsies were performed at the time of death or at the completion of the study. To summarize, the cumulative mortality rate was higher in mice on the HFWD during the 15-month period (55%) than in mice from the low-fat diet or the extract-supplemented high-fat diet groups (20% and 30%, respectively; P < .05 with respect to both). Autopsies revealed colon polyps in 20% of the animals on the HFWD and none in animals of the other 2 groups (P < .05). In addition to the grossly visible polyps, areas of hyperplasia in the colonic mucosa and inflammatory foci throughout the gastrointestinal tract were observed histologically in animals on the high-fat diet. Both were significantly reduced in animals on the low-fat diet and animals on the extract-supplemented HFWD.These data suggest that the mineral-rich algae extract may provide a novel approach to chemoprevention in the colon.
本研究旨在确定一种从红色海藻 Lithothamnion calcareum 中提取的富含矿物质的物质是否可以用作预防结肠癌形成的膳食补充剂。共有 60 只 C57bl/6 小鼠根据饮食分为 3 组。一组接受低脂肪、啮齿动物标准饲料（AIN76A）。第二组接受高脂肪“西式”饮食（HFWD）。第三组接受相同的 HFWD，同时将富含矿物质的提取物作为膳食补充剂。老鼠在各自的饮食上维持 15 个月。在死亡时或研究结束时进行尸检。总的来说，在 15 个月的时间里，高脂肪饮食组的老鼠累积死亡率（55%）高于低脂肪饮食组或提取物补充高脂肪饮食组的老鼠（分别为 20%和 30%；两者均 P <.05）。尸检显示高脂肪饮食组的 20%的动物有结肠息肉，而其他两组的动物均没有（P <.05）。除了肉眼可见的息肉外，高脂肪饮食组动物的结肠黏膜增生区和整个胃肠道的炎症灶在组织学上也有观察到。这些在低脂肪饮食组的动物和在提取物补充的高脂肪饮食组的动物中均显著减少。这些数据表明，富含矿物质的藻类提取物可能为预防结肠癌提供一种新方法。
