Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Calcif Tissue Int. 2010 Apr;86(4):313-24. doi: 10.1007/s00223-010-9340-9. Epub 2010 Feb 24.
The purpose of this study was to determine whether a mineral-rich extract derived from the red marine algae Lithothamnion calcareum could be used as a dietary supplement for prevention of bone mineral loss. Sixty C57BL/6 mice were divided into three groups based on diet: the first group received a high-fat Western-style diet (HFWD), the second group was fed the same HFWD along with the mineral-rich extract included as a dietary supplement, and the third group was used as a control and was fed a low-fat rodent chow diet (AIN76A). Mice were maintained on the respective diets for 15 months. Then, long bones (femora and tibiae) from both males and females were analyzed by three-dimensional micro-computed tomography (micro-CT) and (bones from female mice) concomitantly assessed in bone strength studies. Tartrate-resistant acid phosphatase (TRAP), osteocalcin, and N-terminal peptide of type I procollagen (PINP) were assessed in plasma samples obtained from female mice at the time of sacrifice. To summarize, female mice on the HFWD had reduced bone mineralization and reduced bone strength relative to female mice on the low-fat chow diet. The bone defects in female mice on the HFWD were overcome in the presence of the mineral-rich supplement. In fact, female mice receiving the mineral-rich supplement in the HFWD had better bone structure/function than did female mice on the low-fat chow diet. Female mice on the mineral-supplemented HFWD had higher plasma levels of TRAP than mice of the other groups. There were no differences in the other two markers. Male mice showed little diet-specific differences by micro-CT.
本研究旨在确定从红海藻 Lithothamnion calcareum 中提取的富含矿物质的提取物是否可用作预防骨质流失的膳食补充剂。60 只 C57BL/6 小鼠根据饮食分为三组：第一组接受高脂肪西式饮食（HFWD），第二组在 HFWD 中添加富含矿物质的提取物作为膳食补充剂，第三组作为对照，喂食低脂啮齿动物饲料（AIN76A）。小鼠分别维持在各自的饮食中 15 个月。然后，对雄性和雌性的长骨（股骨和胫骨）进行三维微计算机断层扫描（micro-CT）分析，并同时在骨强度研究中评估雌性小鼠的骨骼。在处死时从雌性小鼠的血浆样本中评估抗酒石酸酸性磷酸酶（TRAP）、骨钙素和 I 型前胶原 N 端肽（PINP）。总之，与低脂饲料组相比，HFWD 组的雌性小鼠骨矿化减少，骨强度降低。在富含矿物质的补充剂存在下，HFWD 组雌性小鼠的骨缺陷得到了克服。事实上，接受 HFWD 中富含矿物质的补充剂的雌性小鼠的骨结构/功能优于低脂饲料组的雌性小鼠。接受 HFWD 中富含矿物质的补充剂的雌性小鼠的血浆 TRAP 水平高于其他组的小鼠。另外两个标志物没有差异。雄性小鼠的微 CT 表现出很少的饮食特异性差异。
