Reichard P
Fed Proc. 1978 Jan;37(1):9-14.
The deoxyribonucleotides required for DNA synthesis are provided by reduction of ribonucleotides. Our studies aim at an understanding of the interplay between the processes involved in the production of deoxyribonucleotides and in their consumption. The enzyme system reducing ribonucleotides has been characterized in some detail, and its regulatory aspects were investigated with pure enzymes. Knowledge gained in this way made it possible to interfere specifically with the activity of ribonucleotide reductase in intact cells and to study effects on DNA synthesis. In this connection, the replication of polyoma DNA was used as a model that allowed dissection of DNA synthesis into different steps involved in the initiation and elongation processes.
DNA合成所需的脱氧核糖核苷酸是通过核糖核苷酸的还原作用提供的。我们的研究旨在了解脱氧核糖核苷酸的产生过程与其消耗过程之间的相互作用。还原核糖核苷酸的酶系统已得到了较为详细的表征，并且利用纯酶对其调节方面进行了研究。通过这种方式获得的知识使得在完整细胞中特异性干扰核糖核苷酸还原酶的活性并研究其对DNA合成的影响成为可能。在此方面，多瘤病毒DNA的复制被用作一个模型，该模型能够将DNA合成解析为起始和延伸过程中涉及的不同步骤。
