Mita M
Department of Biochemistry, Teikyo University School of Medicine, Tokyo, Japan.
Mol Reprod Dev. 1991 Mar;28(3):280-5. doi: 10.1002/mrd.1080280310.
Energy metabolism in spermatozoa of the sea urchin Glyptocidaris crenularis was examined. The spermatozoa contained not only several kinds of phospholipids and cholesterol but also triacylglycerides (TG). Following dilution of the dry sperm in sea water, the TG content decreased rapidly. Other lipids, however, remained at constant levels, except for an increase in the level of free fatty acid. Oil red-O staining of spermatozoa showed that TG was principally located in part of the sperm midpiece. Also, high lipase activity was demonstrated in the spermatozoa. In both intact cells and a cell-free system, 14C-labeled fatty acids were oxidized to 14CO2. It is thus concluded that G. crenularis spermatozoa use TG as a substrate for energy metabolism.
对海胆粗刻疣海胆精子的能量代谢进行了研究。精子不仅含有几种磷脂和胆固醇，还含有甘油三酯（TG）。将干燥的精子在海水中稀释后，TG含量迅速下降。然而，其他脂质保持在恒定水平，除了游离脂肪酸水平有所增加。精子的油红O染色表明，TG主要位于精子中段的一部分。此外，精子中还表现出高脂肪酶活性。在完整细胞和无细胞体系中，14C标记的脂肪酸都被氧化为14CO2。因此得出结论，粗刻疣海胆精子利用TG作为能量代谢的底物。
