海胆精子中用于能量产生的磷脂酰胆碱代谢。

Phosphatidylcholine metabolism for energy production in sea urchin spermatozoa.

作者信息

Mita M, Ueta N

机构信息

Department of Biochemistry, Teikyo University School of Medicine, Tokyo, Japan.

出版信息

Biochim Biophys Acta. 1990 Nov 12;1047(2):175-9. doi: 10.1016/0005-2760(90)90044-x.

DOI:10.1016/0005-2760(90)90044-x

PMID:2248974

Abstract

Sea urchin spermatozoa use endogenous phosphatidylcholine (PC) to produce energy for swimming. The catabolism of PC was studied in Hemicentrotus pulcherrimus spermatozoa. Following incubation in sea water, the content of PC decreased and that of choline increased gradually, whereas phosphocholine maintained a constant level. Measurement of the radioactivity in metabolites converted from 1-palmitoyl-2-[1-14C]linoleoyl-PC, [choline-methyl-14C]dipalmitoyl-PC and 1-[1-14C]palmitoyl-lysophosphatidylcholine (LysoPC) showed that the major degradative pathway is PC----LysoPC----glycerophosphocholine----choline. 1-Palmitoyl-2-[1-14C]linoleoyl-PC and [1-14C]oleic acid were oxidized to 14CO2 in a cell-free system of spermatozoa. Sea urchin spermatozoa thus appear to quite likely obtain energy through the oxidation of fatty acid(s) from PC.

摘要

海胆精子利用内源性磷脂酰胆碱（PC）产生游动所需的能量。对光棘球海胆精子中PC的分解代谢进行了研究。在海水中孵育后，PC的含量降低，胆碱的含量逐渐增加，而磷酸胆碱保持恒定水平。对由1-棕榈酰-2-[1-14C]亚油酰-PC、[胆碱-甲基-14C]二棕榈酰-PC和1-[1-14C]棕榈酰溶血磷脂酰胆碱（溶血PC）转化而来的代谢产物中的放射性进行测量，结果表明主要的降解途径是PC→溶血PC→甘油磷酸胆碱→胆碱。在精子的无细胞体系中，1-棕榈酰-2-[1-14C]亚油酰-PC和[1-14C]油酸被氧化为14CO2。因此，海胆精子似乎很可能通过PC中脂肪酸的氧化来获取能量。