Tessarolo D, Liguori M, Giacanelli M
Service of Neuromuscular Diseases, S. Camillo Hospital, Rome, Italy.
Biochem Med Metab Biol. 1991 Feb;45(1):1-5. doi: 10.1016/0885-4505(91)90002-3.
The NADP(+)-dependent activity of malic enzymes EC 1.1.1.39 and EC 1.1.1.40 was studied in human cardiac and skeletal muscle obtained from living subjects. We used polyacrylamide gel electrophoresis to detect and extract the enzymatic forms and starch gel electrophoresis to confirm their identification. This simple procedure allowed us to provide evidence of a selective NADP(+)-dependent distribution of malic enzyme activities between the two muscular tissues, using a smaller amount of sample than used with previous methods.
