小鼠嵌合体镶嵌骨骼肌中的线粒体苹果酸酶

Mitochondrial malic enzyme in mosaic skeletal muscle of mouse chimeras.

作者信息

Frair P M, Strasberg P M, Freeman K B, Peterson A C

出版信息

Biochem Genet. 1979 Aug;17(7-8):693-702. doi: 10.1007/BF00502127.

Abstract

The question was investigated whether mitochondria in the mammalian skeletal muscle fiber syncytium incorporate gene products encoded by one or many nuclei. Mouse chimeras were produced from strains which differ in their electrophoretic variants of the nuclear-coded mitochondrial protein, malic enzyme (MOD-2, E.C. 1.1.1.40, L-malate NADP+ oxidoreductase decarboxylating). The MOD-2 phenotypes of skeletal muscles of these chimeras were characterized in a starch gel electrophoretic system. The results indicate that individual mitochondria can contain products encoded by multiple nuclei and therefore that, for skeletal muscle mitochondria, the cell is not subdivided into nuclear territories. Possible mechanisms of gene product distribution in skeletal muscle fibers are discussed.

摘要

研究了哺乳动物骨骼肌纤维多核体中的线粒体是否整合由一个或多个细胞核编码的基因产物。通过核编码的线粒体蛋白苹果酸酶（MOD-2，E.C. 1.1.1.40，L-苹果酸NADP+氧化还原酶脱羧酶）电泳变体不同的品系产生小鼠嵌合体。在淀粉凝胶电泳系统中对这些嵌合体骨骼肌的MOD-2表型进行了表征。结果表明，单个线粒体可以包含由多个细胞核编码的产物，因此对于骨骼肌线粒体来说，细胞不会被细分为核区域。讨论了骨骼肌纤维中基因产物分布的可能机制。