Institute of Legal Medicine, Roentgenstr. 23, 48149, Münster, Germany.
Int J Legal Med. 2010 Nov;124(6):559-68. doi: 10.1007/s00414-009-0410-3. Epub 2010 Feb 12.
Do long periods of asphyxiation trigger the proliferation of pulmonary macrophages and the formation of giant cells? Three groups have been defined: six autopsy cases with time periods of suffocation >25 min (long protracted asphyxiation), eight cases with estimated time periods of suffocation 10-25 min (short protracted asphyxiation) and nine cases where death had occurred immediately (very severe trauma). The stain used was haematoxylin and eosin (H&E), and the immunohistochemical stainings were performed using antibodies CD 68, MRP 8, MRP 14 and NP 57. The intraalveolar macrophages and giant cells were counted in H&E sections. For the immunohistochemical stainings, a scoring was used in order to compare the groups. In protracted asphyxiation, the number of intraalveolar macrophages was definitely elevated. A significant increase of giant cells was observed in the cases of long protracted asphyxiation. CD 68 showed clearly elevated numbers in both asphyxiation groups. Early-stage macrophages are significantly increased in protracted asphyxiation. With increasing time periods of asphyxiation, the results become more significant. The results show that the length of the agony period stimulates the proliferation of pulmonary macrophages and the formation of giant cells.
长时间窒息会引发肺泡巨噬细胞增殖和巨细胞形成吗？根据窒息时间的长短，可将研究对象分为三组：六例窒息时间大于 25 分钟（长时间窒息）、八例窒息时间估计为 10-25 分钟（短时间窒息）和九例窒息即刻死亡（严重创伤）。使用的染色方法为苏木精和伊红（H&E）染色，免疫组织化学染色使用的抗体为 CD68、MRP8、MRP14 和 NP57。在 H&E 切片中计算肺泡内巨噬细胞和巨细胞的数量。为了进行组间比较，对免疫组织化学染色进行了评分。在长时间窒息中，肺泡内巨噬细胞的数量明显增加。在长时间窒息的病例中，巨细胞数量明显增加。CD68 在两组窒息中均显示出明显增加的数量。在长时间窒息中，早期的巨噬细胞明显增加。随着窒息时间的延长，结果变得更加显著。结果表明，痛苦期的长短刺激了肺巨噬细胞的增殖和巨细胞的形成。
