Université Paris Descartes, Laboratoire Interaction Gamétique et Fusion des Membranes, EA 2717, Institut Alfred Jost, Service d'Histologie Embryologie Biologie de la Reproduction, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, Paris, France.
Dev Dyn. 2010 Mar;239(3):773-83. doi: 10.1002/dvdy.22206.
Oocyte integrins have been described as essential for fertilization. But this concept has been challenged by deletion experiments. Recently, we have shown that sperm integrin alpha6beta1 plays a determinant role in mouse gamete interaction. In this study, we demonstrate the presence of alphavbeta3 integrin by Western blot and immunofluorescence on the sperm membrane. Oocytes and/or sperm preincubations with anti-alphav or anti-beta3 antibodies were performed before in vitro fertilization on cumulus-intact and zona-free egg assays. We observed inhibitory effects on the fusion process mostly by means of sperm function. An antibody directed against vitronectin inhibited gametes fusion, whereas the presence of exogenous vitronectin increased its efficiency. We suggest that vitronectin (on multimeric forms) can play a first nonspecific link corresponding to loosely bound spermatozoa to oocyte and that this link could be mediated by means of oocyte proteoglycans or integrins, and sperm alphavbeta3 integrin.
卵母细胞整合素被描述为受精所必需的。但这一概念受到了缺失实验的挑战。最近，我们已经证明了精子整合素 alpha6beta1 在小鼠配子相互作用中起着决定性的作用。在这项研究中，我们通过 Western blot 和免疫荧光法在精子膜上证实了 alphavbeta3 整合素的存在。在体外受精前，卵母细胞和/或精子与抗 alphav 或抗 beta3 抗体预孵育，用于有透明带和无透明带卵的实验。我们观察到融合过程受到抑制，主要是通过精子功能。针对 vitronectin 的抗体抑制了配子的融合，而外源性 vitronectin 的存在增加了其效率。我们认为 vitronectin（以多聚体形式）可以发挥第一个非特异性连接，对应于松散结合的精子到卵母细胞，并且这种连接可以通过卵母细胞蛋白聚糖或整合素和精子 alphavbeta3 整合素来介导。
