The Key Laboratory of Luminescence and Realtime Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, No 2 Road Tiansheng, Beibei, Chongqing 400715, China.
Talanta. 2010 Mar 15;80(5):1907-12. doi: 10.1016/j.talanta.2009.10.042. Epub 2009 Oct 24.
The eggshell membrane (ESM) contains several surface functional groups such as amines, amides and carboxylic groups with potential as SPE adsorbent for the retention of target species of interest. In this paper, the potential use of ESM, a typical biomaterial, as solid-phase extraction (SPE) adsorbent is evaluated for analysis of trace arsenic(V) in environmental water samples in combination with hydride generation atomic fluorescence spectrometry (HG-AFS). In order to obtain the satisfactory recovery of arsenic(V), various parameters including the desorption and enrichment conditions such as pH, the flow rate and the volume of sample solution, the amount of ESM and the content of sodium chloride were systematically optimized and the effects of co-existed ions were also investigated in detail. Under the optimal conditions, arsenic(V) could be easily extracted by the ESM packed cartridge and the breakthrough adsorption capacity was found to be 3.9 microg g(-1). The favorable limit of detection (LOD) for arsenic(V) was found to be 0.001 microg L(-1) with an enrichment factor of 33.3, and the relative standard deviations (R.S.Ds) was 2.1% for 0.6 microg L(-1) arsenic (n=11). The reproducibility among columns was satisfactory (R.S.D. among columns is less than 5%). The proposed method has been successfully applied to analysis of arsenic(V) in aqueous environmental samples, which suggests the ESM can be an excellent SPE adsorbent for arsenic(V) pretreatment and enrichment from real water samples.
蛋壳膜（ESM）含有几个表面功能基团，如胺、酰胺和羧酸基团，具有作为 SPE 吸附剂保留目标感兴趣物种的潜力。在本文中，评估了典型生物材料 ESM 作为固相萃取（SPE）吸附剂在与氢化物发生原子荧光光谱法（HG-AFS）结合分析环境水样中痕量砷（V）中的潜在用途。为了获得令人满意的砷（V）回收率，系统地优化了各种参数，包括洗脱和富集条件，如 pH 值、样品溶液的流速和体积、ESM 的用量和氯化钠的含量，并详细研究了共存离子的影响。在最佳条件下，ESM 填充柱可轻松提取砷（V），突破吸附容量为 3.9 µg g(-1)。砷（V）的有利检出限（LOD）为 0.001 µg L(-1)，富集因子为 33.3，对于 0.6 µg L(-1) 砷，相对标准偏差（RSD）为 2.1%（n=11）。柱子之间的重现性令人满意（柱子之间的 RSD 小于 5%）。该方法已成功应用于水样中砷（V）的分析，表明 ESM 可以作为从实际水样中预处理和富集砷（V）的优秀 SPE 吸附剂。
