利用 2,4-二羟基-3-碘水杨醛嗪实现对水溶液中 HSA/BSA 的简便、灵敏、选择性荧光开启检测。

Facile, sensitive and selective fluorescence turn-on detection of HSA/BSA in aqueous solution utilizing 2,4-dihydroxyl-3-iodo salicylaldehyde azine.

机构信息

Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, PR China.

出版信息

Talanta. 2010 Mar 15;80(5):1952-8. doi: 10.1016/j.talanta.2009.10.053. Epub 2009 Nov 1.

DOI:10.1016/j.talanta.2009.10.053

PMID:20152438

Abstract

A novel fluorescence turn-on detection method of human serum albumin (HSA) and bovine serum albumin (BSA) in aqueous solution is investigated using 2,4-dihydroxyl-3-iodo salicylaldehyde azine (DISA). Upon the addition of DISA to HSA/BSA solution, a fluorescence turn-on effect at 529 nm can be observed with a large stokes shift of approximately 129 nm based on hydrophobic binding-mode between protein and dye. Under the optimal condition, the linear ranges of fluorescence intensity for HSA and BSA are 0.1-30 microg mL(-1) with the relative correlation coefficient of R(2)=0.991 (n=10) and 0.3-50 microg mL(-1) with R(2)=0.997 (n=10); and the detection limits for HSA and BSA based on IUPAC (C(DL)=3S(b)/m) are 20 ng mL(-1) and 50 ng mL(-1), respectively.

摘要

研究了一种新型荧光开启检测法，用于在水溶液中检测人血清白蛋白（HSA）和牛血清白蛋白（BSA），所用试剂为 2,4-二羟基-3-碘水杨醛嗪（DISA）。向 HSA/BSA 溶液中加入 DISA 后，可观察到在 529nm 处的荧光开启效应，其斯托克斯位移约为 129nm，这是基于蛋白质与染料之间的疏水结合模式。在最佳条件下，HSA 和 BSA 的荧光强度线性范围分别为 0.1-30μgmL(-1)，相对相关系数 R(2)=0.991（n=10）和 0.3-50μgmL(-1)，R(2)=0.997（n=10）；根据 IUPAC（C(DL)=3S(b)/m），HSA 和 BSA 的检测限分别为 20ngmL(-1)和 50ngmL(-1)。