Tyndall National Institute, Lee Maltings, University College Cork, Cork, Ireland.
Talanta. 2010 Mar 15;80(5):1993-8. doi: 10.1016/j.talanta.2009.10.060. Epub 2009 Nov 10.
In this work, the effect of the serum protein, bovine serum albumin (BSA), on the detection of propranolol in artificial serum by ion-transfer voltammetry at an array of micro-interfaces between two immiscible electrolyte solutions (microITIES) is presented. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and differential pulse stripping voltammetry (DPSV) were examined for the detection of low concentrations of propranolol. Both CV and DPV had an interference effect from BSA, manifested as lower currents in the presence of the protein. DPSV proved to be the most effective technique, enabling the detection of 0.05 microM propranolol in the presence of BSA. The DPSV method employed a preconditioning step as well as a preconcentration step followed by the analytical signal generation step. The latter was based on the back-transfer of the drug across the microITIES. The preconcentration step was crucial to prevention of the adverse effects of BSA on the voltammetric detection. These results demonstrate that serum-protein effects on drug detection at low concentrations can be eliminated by use of DPSV at arrays of microITIES. CVs of propranolol with increasing concentrations of BSA revealed the influence of the drug-protein binding interaction, with decreases in current but no change in transfer potential. Therapeutic concentrations of propranolol were detected, demonstrating the viability of this approach for bioanalytical investigations.
在这项工作中，研究了血清蛋白牛血清白蛋白（BSA）对在两个不混溶电解质溶液（microITIES）之间的微界面阵列中通过离子转移伏安法检测人工血清中普萘洛尔的影响。循环伏安法（CV）、差分脉冲伏安法（DPV）和差分脉冲溶出伏安法（DPSV）被用于检测低浓度的普萘洛尔。CV 和 DPV 都受到 BSA 的干扰，表现为存在蛋白质时电流较低。DPSV 被证明是最有效的技术，能够在存在 BSA 的情况下检测到 0.05 μM 的普萘洛尔。DPSV 方法采用了预处理步骤以及预浓缩步骤，然后是分析信号生成步骤。后者基于药物在 microITIES 之间的反向转移。预浓缩步骤对于防止 BSA 对伏安检测的不利影响至关重要。这些结果表明，通过在 microITIES 阵列中使用 DPSV 可以消除血清蛋白对低浓度药物检测的影响。随着 BSA 浓度的增加，普萘洛尔的 CV 揭示了药物-蛋白质结合相互作用的影响，电流减小但传递电位没有变化。检测到治疗浓度的普萘洛尔，证明了这种方法用于生物分析研究的可行性。
