Department of Chemistry, School of Science, Alzahra University, P.O. Box 1993891176, Tehran, Iran.
Bioelectrochemistry. 2009 Nov;77(1):26-30. doi: 10.1016/j.bioelechem.2009.05.011. Epub 2009 May 30.
The electrochemical behavior of the interaction of furazolidone (Fu) with bovine serum albumin (BSA) was investigated by cyclic voltammetry and differential pulse voltammetry at a glassy carbon electrode. Fu shows an irreversible reduction at -0.34 V in pH 4.0 Britton-Robinson buffer (B-R) buffer-10% DMF solution. After the addition of BSA into the Fu solution, the reductive peak currents decreased without any significant shift of the peak potential and the appearance of new peaks. The electrochemical parameters of the interaction system were calculated in the absence and presence of BSA. This electrochemical method was further applied to the determination of BSA samples and the results were in good agreement with the traditional cellulose acetate electrophoresis. The linear dynamic range was between 10.0 and 80.0 mg l(-1). The detection limit was 7.6 mg l(-1) and the recoveries were obtained from 97.0% to 104.0%.
采用循环伏安法和差分脉冲伏安法在玻碳电极上研究了呋喃唑酮（Fu）与牛血清白蛋白（BSA）相互作用的电化学行为。在 pH 4.0 的 Britton-Robinson 缓冲液（B-R）-10%DMF 溶液中，Fu 在-0.34 V 处显示出不可逆的还原峰。在 Fu 溶液中加入 BSA 后，还原峰电流减小，而峰电位没有明显移动，也没有出现新的峰。在不存在和存在 BSA 的情况下计算了相互作用体系的电化学参数。该电化学方法进一步应用于 BSA 样品的测定，结果与传统的醋酸纤维素电泳法吻合良好。线性动态范围为 10.0 至 80.0mg l(-1)。检测限为 7.6mg l(-1)，回收率为 97.0%至 104.0%。
