Fuda Cancer Hospital at Guangzhou, China; The GIBH Affiliated Fuda Hospital, Chinese Academy of Sciences, China.
Cryobiology. 2010 Jun;60(3):281-6. doi: 10.1016/j.cryobiol.2010.01.006. Epub 2010 Feb 10.
This study was designed to basic information concerning the efficacy and safety of cryosurgery for pancreatic cancer. Fifteen healthy pigs were used to perform biochemical analysis and histological assessment.
Following anesthesia and laparotomy, an argon-helium cryoprobe was inserted into the pancreas. The introduction of argon gas induced a rapid decrease in temperature to -160 degrees C (Group I, 5 pigs) or -110 degrees C (Group II, 5 pigs), respectively, resulting in ice-ball formation of 15-20mm diameter after 5 min. Following freezing, helium gas was circulated in the probe tip to increase the temperature to 10-20 degrees C over 3 min to thaw. The freeze/thaw cycle was then repeated. Group III (3 pigs) had a cryoprobe inserted, but without freezing, and Group IV (2 pigs) included untreated or normal control animals. Levels of serum amylase (AMY), IL-6 and C-RP were measured prior to freezing and for 7 days following the procedure. All pigs were euthanized 7 days post-treatment and pancreases were examined histologically.
Neither hyperaemia, edema or hemorrhage were observed in the un-frozen parts of the pancreas. Histological assessment revealed a significant level of necrosis in the central and lateral regions of the tissue frozen within the ice-ball. All cellular ultrastructure was destroyed and only observable as a few of remaining nuclei with broken crests and degranulated mitochondria and rough endoplasmic reticulum. There was a significant increase of serum AMY levels for a brief period in both "deep frozen" and the "shallow frozen" groups. However, the AMY also increased in two pigs in the "normal control" group and one pig from the "inserted cryoprobe without freeze" control group. All experimental pigs appeared healthy until the sacrifice time.
Cryosurgery is a safe and effective ablative procedure for pancreatic tissue resulting in minimal complications.
本研究旨在获取有关胰腺癌冷冻治疗疗效和安全性的基本信息。我们使用 15 头健康猪进行了生化分析和组织学评估。
麻醉并剖腹后，将氩氦冷冻探针插入胰腺。引入氩气会导致温度迅速降至-160°C（I 组，5 头猪）或-110°C（II 组，5 头猪），5 分钟后形成 15-20mm 直径的冰球。冷冻后，氦气在探针尖端循环，将温度在 3 分钟内升高到 10-20°C 以解冻。然后重复冷冻/解冻循环。III 组（3 头猪）插入冷冻探针但不进行冷冻，IV 组（2 头猪）包括未经处理或正常对照动物。在冷冻前和冷冻后 7 天测量血清淀粉酶（AMY）、IL-6 和 C-RP 水平。所有猪在治疗后 7 天安乐死，并检查胰腺的组织学。
未冷冻部分的胰腺既无充血、水肿也无出血。组织学评估显示，在冰球内冷冻的组织的中央和侧区有明显的坏死程度。所有细胞超微结构均被破坏，仅可见少数残留核，嵴破裂、脱粒的线粒体和粗面内质网。“深度冷冻”和“浅层冷冻”两组的血清 AMY 水平在短时间内均显著升高。然而，“正常对照”组的 2 头猪和“插入冷冻探针但不冷冻”对照组的 1 头猪的 AMY 也升高了。所有实验猪在安乐死前均表现健康。
冷冻治疗是一种安全有效的胰腺组织消融方法，并发症少。
