Department of Diagnostic Radiology, School of Medicine, KEIO University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Cryobiology. 2010 Dec;61(3):317-26. doi: 10.1016/j.cryobiol.2010.10.157. Epub 2010 Oct 29.
Regarding cryoablation for the malignant lung tumors, multiple trials of the freeze-thaw process have been made, and we considered it necessary to view and analyze the freeze-thaw process as a freeze-thaw sequence. We caused the sequence in a porcine lung in vivo by using an acicular, cylindrical stainless-steel probe as the heat source for the freeze-thaw sequence and cooling to -150 °C with super high-pressure argon gas by causing the Joule-Thomson effect phenomenon at the tip of the probe. In this experiment, we examined the sequence by measuring the temperature and using the isothermal curve and the freezing function. As a result, it was demonstrated that the freezing characteristics considerably differed in the first sequence and the second sequence from those of non-aerated organs such as liver and kidney. In our experiments on porcine lung, thermal properties were considered to change as the bleeding caused by the first thawing infiltrated in the lung parenchyma, and it was confirmed that the frozen area in the second cycle was dramatically enlarged as compared with the first cycle (when a similar sequence is continuously repeated, we say it as cycle). This paper provides these details.
关于冷冻消融治疗恶性肺肿瘤，已经进行了多次冷冻-融化过程的试验，我们认为有必要将冷冻-融化过程视为一个冷冻-融化序列进行观察和分析。我们使用针状圆柱形不锈钢探头作为冷冻-融化序列的热源，通过在探头尖端产生焦耳-汤姆逊效应现象，将超高压氩气冷却至-150°C，在活体猪肺中引发了该序列。在这个实验中，我们通过测量温度和使用等温曲线和冻结功能来检查序列。结果表明，与肝脏和肾脏等非充气器官相比，第一序列和第二序列的冻结特性有很大的不同。在我们对猪肺的实验中，考虑到第一次融化引起的出血渗透到肺实质中，热特性可能会发生变化，并且证实与第一周期相比（当连续重复类似序列时，我们称之为周期），第二周期的冻结区域显著扩大。本文提供了这些细节。
