Recombinant DNA Laboratory, Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, Uttar Pradesh, India.
J Virol Methods. 2010 May;165(2):277-82. doi: 10.1016/j.jviromet.2010.02.008. Epub 2010 Feb 10.
The routine technique for detecting antibodies specific to infectious bursal disease virus is a serological evaluation by enzyme linked immunosorbent assay (ELISA) with preparations of whole virions as antigens. To avoid the use of complete virus in the standard technique, in-house VP2 and VP3 based ELISAs were developed. Accordingly, four types of indirect ELISAs viz., a commercial IDEXX-ELISA kit, VP2 and or VP3 antigen based ELISAs and a whole virus ELISA were compared with the virus neutralization test. It was concluded that the sensitivity and specificity at receiver-operating characteristics (ROC) optimized cut-off of four ELISAs viz., IDEXX-ELISA, VP2-ELISA and VP3-ELISA indicated similar performance whereas whole virus antigen based ELISA showed poor performance in comparison to other ELISAs. Similarly the positive and negative likelihood ratio of four ELISAs at an optimized cut-off indicated IDEXX-ELISA to be the best among all the four ELISAs while the performance of rVP3-ELISA and rVP2-ELISA is good as compared to the whole virus ELISA. Finally, the area under the ROC curve (AUC) of four ELISAs which represented a summary statistics of the overall diagnostic performance of the test also indicated that the IDEXX-ELISA, VP3-ELISA and VP2-ELISA had similar and relatively better performance when compared to whole virus antigen-ELISA.
检测传染性法氏囊病病毒特异性抗体的常规技术是通过酶联免疫吸附试验（ELISA）用全病毒制剂作为抗原进行血清学评估。为避免在标准技术中使用完整病毒，开发了基于 VP2 和 VP3 的内部 ELISA。因此，将四种间接 ELISA（即商业 IDEXX-ELISA 试剂盒、基于 VP2 和/或 VP3 抗原的 ELISA 和全病毒 ELISA）与病毒中和试验进行了比较。结论是，在接收者操作特征（ROC）优化截断值下，四种 ELISA（IDEXX-ELISA、VP2-ELISA 和 VP3-ELISA）的敏感性和特异性表明相似的性能，而基于全病毒抗原的 ELISA 与其他 ELISA 相比表现不佳。同样，在优化截断值下，四种 ELISA 的阳性和阴性似然比表明 IDEXX-ELISA 在所有四种 ELISA 中表现最佳，而 rVP3-ELISA 和 rVP2-ELISA 的性能与全病毒 ELISA 相比较好。最后，ROC 曲线下面积（AUC）代表测试整体诊断性能的汇总统计数据，也表明 IDEXX-ELISA、VP3-ELISA 和 VP2-ELISA 与全病毒抗原-ELISA 相比具有相似且相对较好的性能。
