Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russian Federation.
K. I. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow, Russian Federation.
Arch Virol. 2020 Jul;165(7):1611-1620. doi: 10.1007/s00705-020-04650-2. Epub 2020 May 13.
Infectious bursal disease virus (IBDV), which infects young chickens, is one of the most important pathogens that harm the poultry industry. Evaluation of the immune status of birds before and after vaccination is of great importance for controlling the disease caused by this virus. Therefore, the development of low-cost and easy-to-manufacture test systems for IBDV antibody detection remains an urgent issue. In this study, three expression systems (bacteria, yeast, and human cells) were used to produce recombinant VP3 protein of IBDV. VP3 is a group-specific antigen and hence may be a good candidate for use in diagnostic tests. Comparison of the antigenic properties of the obtained polypeptides showed that the titres of antibodies raised in chickens against bacteria- or human-cell-derived recombinant VP3 were high, whereas the antibody level against yeast-derived recombinant VP3 was low. The results of an enzyme-linked immunosorbent assay (ELISA) of sera from IBDV-infected chickens demonstrated that the recombinant VP3 produced in E. coli would be the best choice for use in test systems.
传染性腔上囊病病毒（IBDV）可感染雏鸡，是危害家禽业的最重要病原体之一。评估禽类接种疫苗前后的免疫状态对于控制该病毒引起的疾病非常重要。因此，开发用于 IBDV 抗体检测的低成本、易于制造的测试系统仍然是一个紧迫的问题。在这项研究中，使用了三种表达系统（细菌、酵母和人细胞）来生产 IBDV 的重组 VP3 蛋白。VP3 是一种群特异性抗原，因此可能是诊断测试的良好候选物。对获得的多肽的抗原特性进行比较表明，针对细菌或人细胞衍生的重组 VP3 产生的鸡抗体的效价很高，而针对酵母衍生的重组 VP3 的抗体水平较低。IBDV 感染鸡血清的酶联免疫吸附试验（ELISA）结果表明，在大肠杆菌中产生的重组 VP3 将是用于测试系统的最佳选择。
