School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China.
Bioresour Technol. 2010 Jun;101(12):4508-12. doi: 10.1016/j.biortech.2010.01.073. Epub 2010 Feb 12.
Coupling bio-production of hydrogen and polyhydroxyalkanoates (PHA) from Taihu blue algae through metabolites circulation was investigated. It was found that the pH adjustment, especially basification was more practical and efficient than other methods for the pretreatment of blue algae before anaerobic digestion. On this occasion, SCOD, biogas accumulation and hydrogen content reached 26 mg/gTS, 500 mL and 37.2%, and which were 4.3, 1.3 and 14.4 times of those of the control group, respectively. Secondly, amounts of both butyric acid and hydrogen could be further increased when blue algae was alkali pretreated at pH 13, as the accumulation of butyric acid, acetic acid and hydrogen reached 1.7, 1.4 and 3.8 times compared to those of the control, respectively. Finally, the coupling bio-production of hydrogen and PHA was conducted through pumping organic residues into PHA fermenter from anaerobic digester. Remarkably, it was found that the larger the pumping rate of carbon and nitrogen sources supply, the higher the yield of DCW and PHA could be expected by Bacillus cereus.
通过代谢物循环，研究了从太湖蓝藻中生物生产氢气和聚羟基烷酸（PHA）的耦合。结果发现，与其他预处理方法相比，在厌氧消化前对蓝藻进行 pH 调节，特别是碱化处理更实用、更有效。在此情况下，SCOD、沼气积累和氢气含量分别达到 26mg/gTS、500mL 和 37.2%，分别是对照组的 4.3、1.3 和 14.4 倍。其次，当蓝藻在 pH 13 下进行碱预处理时，丁酸和氢气的含量均可进一步增加，与对照组相比，丁酸、乙酸和氢气的积累分别达到 1.7、1.4 和 3.8 倍。最后，通过从厌氧消化器向 PHA 发酵器泵送有机残留物来进行氢气和 PHA 的耦合生物生产。值得注意的是，发现通过芽孢杆菌泵送碳氮源的速率越大，预期 DCW 和 PHA 的产量就越高。
