Patel Sanjay K S, Lee Jung-Kul, Kalia Vipin C
Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi, 110007 India ; Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 143-701 Korea.
Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 143-701 Korea.
Indian J Microbiol. 2016 Sep;56(3):293-300. doi: 10.1007/s12088-016-0595-3. Epub 2016 May 10.
In this study, an integrative approach to produce biohydrogen (H2) and polyhydroxyalkanoates (PHA) from the wastes of biological origin was investigated. A defined set of mixed cultures was used for hydrolysis and the hydrolysates were used to produce H2. The effluent from H2 production stage was used for PHA production. Under batch culture, a maximum of 62 l H2/kg of pure potato peels (Total solid, TS 2 %, w/v) and 54 l H2/kg of mixed biowastes (MBW1) was recorded. Using effluent from the H2 production stage of biowaste mixture (MBW1), Bacillus cereus EGU43 could produce 195 mg PHA/l and 15.6 % (w/w). Further, supplementation of GM-2 medium (0.1×) and glucose (0.5 %) in H2 production stage effluents, resulted in significant improvements of up to 11 and 41.7 % of PHA contents, respectively. An improvement of 3.9- and 17-fold in PHA yields as compared to with and without integrative H2 production from the MBW1 has been recorded. This integrative approach seems to be a suitable process to improve the yields of H2 and PHA by mixing biowastes.
在本研究中，研究了一种从生物源废弃物中生产生物氢（H₂）和聚羟基脂肪酸酯（PHA）的综合方法。使用一组特定的混合培养物进行水解，并将水解产物用于生产H₂。H₂生产阶段的流出物用于生产PHA。在分批培养条件下，记录到每千克纯土豆皮（总固体，TS 2%，w/v）最多可产生62升H₂，每千克混合生物废弃物（MBW1）最多可产生54升H₂。利用生物废弃物混合物（MBW1）H₂生产阶段的流出物，蜡样芽孢杆菌EGU43可产生195毫克/升PHA，含量为15.6%（w/w）。此外，在H₂生产阶段的流出物中添加GM-2培养基（0.1×）和葡萄糖（0.5%），分别使PHA含量显著提高了11%和41.7%。与MBW1单独生产H₂和不进行H₂综合生产相比，PHA产量分别提高了3.9倍和17倍。这种综合方法似乎是一种通过混合生物废弃物来提高H₂和PHA产量的合适工艺。
