REQUIMTE/CQFB, Chemistry Dpt., FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Bioresour Technol. 2013 Mar;132:146-53. doi: 10.1016/j.biortech.2013.01.050. Epub 2013 Jan 18.
For the first time, a mixed photosynthetic culture (MPC) consisting of a consortium of bacteria and algae was investigated for its capacity to accumulate polyhydroxyalkanoates (PHA). The culture was subjected to a feast and famine regime in an illuminated environment without supplying oxygen or any other electron acceptor. The MPC accumulated PHA during the feast phase and consumed it in the famine phase, where the PHA consumption was made possible due to oxygen production by algae. The internal cycling of carbohydrates was also observed, which was likely linked to bacterial glycogen being used as an additional source of energy for acetate uptake during the feast phase, and restored in the famine phase via PHA degradation. The MPC reached a PHA content of 20%, with a PHA storage yield per acetate similar to aerobic systems, opening up the possibility of a new sunlight-driven PHA production process without the need for aeration.
首次研究了一种由细菌和藻类组成的混合光合培养物（MPC）积累聚羟基烷酸酯（PHA）的能力。该培养物在光照环境中经历了饱食和饥饿阶段，在此期间不提供氧气或任何其他电子受体。MPC 在饱食阶段积累 PHA，并在饥饿阶段消耗 PHA，其中 PHA 的消耗是由于藻类产生氧气而成为可能的。还观察到了碳水化合物的内部循环，这可能与细菌糖原在饱食阶段被用作额外的乙酸摄取能源有关，并且在饥饿阶段通过 PHA 降解得以恢复。MPC 达到了 20%的 PHA 含量，每乙酸的 PHA 储存产率与好氧系统相似，为无需通气的新型阳光驱动 PHA 生产过程开辟了可能性。
