Biosystems Department, Risø National Laboratory for Sustainable Energy, Technical University of Denmark, 4000 Roskilde, Denmark.
Trends Plant Sci. 2010 Apr;15(4):196-203. doi: 10.1016/j.tplants.2010.01.005. Epub 2010 Feb 10.
Increasing evidence shows that many RNAs are targeted to specific locations within cells, and that RNA-processing pathways occur in association with specific subcellular structures. Compartmentation of mRNA translation and RNA processing helps to assemble large RNA-protein complexes, while RNA targeting allows local protein synthesis and the asymmetric distribution of transcripts during cell polarisation. In plants, intercellular RNA trafficking also plays an additional role in plant development and pathogen defence. Methods that allow the visualisation of RNA sequences within a cellular context, and preferably at subcellular resolution, can help to answer important questions in plant cell and developmental biology. Here, we summarise the approaches currently available for localising RNA in vivo and address the specific limitations inherent with plant systems.
越来越多的证据表明，许多 RNA 被靶向到细胞内的特定位置，并且 RNA 处理途径与特定的亚细胞结构相关联。mRNA 翻译和 RNA 处理的分隔有助于组装大型 RNA-蛋白质复合物，而 RNA 靶向则允许在细胞极化过程中进行局部蛋白质合成和转录本的不对称分布。在植物中，细胞间 RNA 运输也在植物发育和病原体防御中发挥额外的作用。能够在细胞内环境中可视化 RNA 序列的方法，最好是在亚细胞分辨率下，有助于回答植物细胞和发育生物学中的重要问题。在这里，我们总结了目前用于体内定位 RNA 的方法，并解决了植物系统固有的特定限制。
