The Mina & Everard Goodman Faculty of Life Sciences & Institute of Nanotechnology, Bar-Ilan University, Ramat Gan, Israel.
Nucleus. 2013 May-Jun;4(3):195-205. doi: 10.4161/nucl.24434. Epub 2013 Apr 11.
The passage of mRNA molecules from the site of synthesis, through the nucleoplasm and the nuclear pore, en route to the cytoplasm, might appear straightforward. Nonetheless, several decades of detailed examination of this pathway, from high resolution electron microscopy in fixed specimens, through the development of immuno-detection techniques and fluorescence toolkits, to the current era of live-cell imaging, show this to be an eventful journey. In addition to mRNAs, several species of noncoding RNAs travel and function in the nucleus, some being retained within throughout their lifetime. This review will highlight the nucleoplasmic paths taken by mRNAs and noncoding RNAs in eukaryotic cells with special focus on live-cell data and in concurrence with the biophysical nature of the nucleus.
mRNA 分子从合成部位通过核质和核孔进入细胞质的过程看似简单，但经过几十年的详细研究，包括利用高分辨率电子显微镜观察固定标本，开发免疫检测技术和荧光工具，以及当前的活细胞成像时代，我们发现这是一个充满事件的旅程。除了 mRNA，还有几种非编码 RNA 在细胞核中运输和发挥作用，其中一些在其整个生命周期内都被保留在细胞核内。本综述将重点介绍真核细胞中 mRNA 和非编码 RNA 的核质途径，特别关注活细胞数据，并与细胞核的物理性质相一致。
