Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
FEBS Lett. 2010 Mar 19;584(6):1156-62. doi: 10.1016/j.febslet.2010.02.018. Epub 2010 Feb 12.
Proteins with Pumilio RNA binding domains (Puf proteins) are ubiquitous in eukaryotes. Some Puf proteins bind to the 3'-untranslated regions of mRNAs, acting to repress translation and promote degradation; others are involved in ribosomal RNA maturation. The genome of Trypanosoma brucei encodes eleven Puf proteins whose function cannot be predicted by sequence analysis. We show here that epitope-tagged TbPUF7 is located in the nucleolus, and associated with a nuclear cyclophilin-like protein, TbNCP1. RNAi targeting PUF7 reduced trypanosome growth and inhibited two steps in ribosomal RNA processing.
具有 Pumilio RNA 结合结构域（Puf 蛋白）的蛋白质在真核生物中普遍存在。一些 Puf 蛋白结合到 mRNA 的 3'-非翻译区，起到抑制翻译和促进降解的作用；其他的则参与核糖体 RNA 的成熟。布氏锥虫的基因组编码十一种 Puf 蛋白，它们的功能不能通过序列分析来预测。我们在这里表明，表位标记的 TbPUF7 位于核仁中，并与一种核环孢菌素样蛋白 TbNCP1 相关。针对 PUF7 的 RNAi 降低了锥虫的生长并抑制了核糖体 RNA 加工的两个步骤。
