Department of Neuroscience and CIBERNED, Vizcaya Technology Park, Neurotek-UPV/EHU, E-48170 Zamudio, Vizcaya, Spain.
J Neurosci Methods. 2010 May 15;188(2):205-12. doi: 10.1016/j.jneumeth.2010.02.008. Epub 2010 Feb 11.
Functional and reliable in vitro models of Parkinson's disease (PD) are valuable for studying mechanisms of dopaminergic degeneration before proceeding to animal testing. At present, all in vitro models involve substitute cell types and thus their direct relevance to PD is questionable. Here, we describe an organotypic culture model which conserves the 3D architecture of the nigro-striatal pathway, together with the subventricular zone and cerebral cortex, and recapitulates a specific pattern of dopaminergic degeneration which is the principal hallmark of PD. The organotypic culture is kept in vitro for up to 12 days and dopaminergic degeneration is induced by the simple cutting of dopaminergic fibers. This organotypic model represents a rapid and useful method (30 min/pup for preparation and up to 12 days of cultivation) to investigate in vitro the mechanisms underlying neuronal death and protection, as well as neurogenesis and repair after nigro-striatal neurodegeneration.
帕金森病（PD）的功能可靠的体外模型对于在进行动物试验之前研究多巴胺能变性的机制非常有价值。目前，所有的体外模型都涉及替代细胞类型，因此它们与 PD 的直接相关性值得怀疑。在这里，我们描述了一种器官型培养模型，该模型保留了黑质纹状体通路的 3D 结构，以及脑室下区和大脑皮层，并再现了多巴胺能变性的特定模式，这是 PD 的主要标志。器官型培养在体外保持长达 12 天，并通过简单地切割多巴胺能纤维来诱导多巴胺能变性。这种器官型模型代表了一种快速有用的方法（每个幼崽的制备时间为 30 分钟，培养时间长达 12 天），可用于体外研究黑质纹状体神经退行性变后神经元死亡和保护以及神经发生和修复的机制。
